Source: https://tendernation.io/opportunities/01a13c8f-eed2-73e7-bd43-31280d39ec6a/cefas22-26-rfq-for-dna-extraction-metabarcoding-and-bioinformatics-of-marine-e awarded GB open procedure Suitable for SMEs # CEFAS22-26 RFQ for DNA extraction, metabarcoding, and bioinformatics of marine environmental samples for Atlantic Bluefin Tuna (ABFT) [Defra Network eTendering Portal](https://tendernation.io/buyers/defra-network-etendering-portal) Often single bidder Estimated value £20,880 Published 20 Apr 2022 Deadline Closed 12 Apr 2022 ## Who can bid - Buyer says suitable for SMEs The buyer ticked “suitable for SMEs” on the notice. Anyone can still bid. - Small contract value Valued under £135,018 including VAT, the lowest Procurement Act threshold for goods and services. - Open procedure Anyone can submit a full tender; there is no shortlisting stage. [More contracts for small businesses and VCSEs](https://tendernation.io/opportunities/for-small-businesses) ## About this tender Cefas has contracted the supplier to provide services for DNA to be extracted from 116 frozen Sterivex cartridges (Millipore; SVGP01050). The cartridges consist of 29 samples in triplicate, and 29 field blanks. Following extraction, Cefas require half the extracted DNA to be sent back to the Cefas laboratory in Lowestoft so we can undertake qPCR (e.g., each of the extracted samples will be halved, one half will be used for metabarcoding, while the other half will be used for qPCR). Cefas require the extracted DNA to undergo metabarcoding using the MiFish 12s marker (Miya et al., 2015) (and MiFish T markers if dual amplicon approach can be achieved). The supplier will also conduct taxonomic assignment on the metabarcoding data using amplicon sequence variants (preferred) or operational taxonomic units and Blast on a Genbank generated database (or similar). Metabarcoding results should be presented to Cefas so that the number of sequences per taxa is available for every sample in a table format. The supplier will also provide the raw data for the sequencing run and methodology involved. Cefas will report results in a scientific manuscript, in consequence details on the methodology provided by the supplier will need to be sufficient to pass peer review (proprietary techniques will be protected). The supplier will provide weekly updates via email detailing progress on deliverables. The supplier will provide all deliverables within 10 weeks of Contract start date. Read full description ## CPV classification Analysis services Source Contracts Finder (UK) [View official notice](https://www.contractsfinder.service.gov.uk/notice/969c0485-1424-47ee-b548-d4a273b74851) Never miss a tender like this again Get AI summaries, deadline alerts and a bid pipeline for tenders matched to your business. Free to start. 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