Source: https://tendernation.io/opportunities/01a13c8f-a742-704a-8015-d78a461cd820/rbgkew1066-long-read-ash-genome-sequencing awarded GB open procedure Suitable for SMEs # RBGKEW1066 Long-Read Ash Genome Sequencing [Board of Trustees of the Royal Botanic Gardens Kew](https://tendernation.io/buyers/board-of-trustees-of-the-royal-botanic-gardens-kew) Often single bidder Estimated value £57,000 Published 21 Jun 2022 Deadline Closed 19 Jul 2022 ## Who can bid - Buyer says suitable for SMEs The buyer ticked “suitable for SMEs” on the notice. Anyone can still bid. - Small contract value Valued under £135,018 including VAT, the lowest Procurement Act threshold for goods and services. - Open procedure Anyone can submit a full tender; there is no shortlisting stage. [More contracts for small businesses and VCSEs](https://tendernation.io/opportunities/for-small-businesses) ## About this tender RBG Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 50 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior has a 1C genome size of 880 Mbp. RBG Kew intends to supply total genomic DNA for each tree with the following metrics: minimum of 20ug DNA; minimum concentration of 30ng/ul (as measured with a Quantus flurometer dsDNA assay); at least 80% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.7; minimum OD260/230 of 1.7. Based on a fixed budget of £57,000 GBP (excluding VAT), we wish to maximise: firstly, the number of trees sequenced at a minimum of 15x whole genome coverage (minimum of 13.2 gigabases of data per sample); secondly, in the event that all 50 trees can be sequenced to at least 15x (minimum of 13.2 gigabases of data per sample), the total amount of data in gigabases for each of the 50 trees. You are hereby invited by the Board of Trustees of the Royal Botanic Gardens, Kew to submit a tender to meet the Customer's requirements in accordance with the specification and associated documents as contained within this tender pack. Tender documents can be accessed via: [defra.bravosolution.co.uk](https://defra.bravosolution.co.uk) [defra.bravosolution.co.uk/go/08518853018185EF98F9](https://defra.bravosolution.co.uk/go/08518853018185EF98F9) itt\_10303 project\_36622 ### Title:- RBGKEW1066 Long-Read Ash Genome Sequencing 2022 Suppliers will be required to register with BRAVO, if not already registered. All tender documents can only be accessed via BRAVO Additional information: Tender documents can be accessed via: [defra.bravosolution.co.uk](https://defra.bravosolution.co.uk) [defra.bravosolution.co.uk/go/08518853018185EF98F9](https://defra.bravosolution.co.uk/go/08518853018185EF98F9) itt\_10303 project\_36622 ### Title: RBGKEW1066 Long-Read Ash Genome Sequencing 2022 Suppliers will be required to register with BRAVO if not already registered. All tender documents can only be accessed via BRAVO Read full description ## CPV classification Laboratory services Source Contracts Finder (UK) [View official notice](https://www.contractsfinder.service.gov.uk/notice/44ca7f5e-f055-41b6-a510-adbd3af12b71) Never miss a tender like this again Get AI summaries, deadline alerts and a bid pipeline for tenders matched to your business. Free to start. 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