Source: https://tendernation.io/opportunities/01a13c38-6866-7341-a153-1fb1e66b7a19/provision-of-single-cell-western-blot-equipment

awarded GB open procedure Suitable for SMEs

# Provision of single cell western blot equipment

[Public Health England](https://tendernation.io/buyers/public-health-england)

Estimated value

£25,000

Published

22 Dec 2019

Deadline

Closed

19 Dec 2019

## Who can bid

- Buyer says suitable for SMEs

    The buyer ticked “suitable for SMEs” on the notice. Anyone can still bid.
- Small contract value

    Valued under £135,018 including VAT, the lowest Procurement Act threshold for goods and services.
- Open procedure

    Anyone can submit a full tender; there is no shortlisting stage.

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## About this tender

Public Health England (PHE) awarded a contract for provision of single cell western blot equipment to Bio-Techne Ltd.

The equipment will be used PHE Centre for Radiation, Chemical and Environmental Hazards (CRCE) in Chilton to research the processes underpinning radiation-induced cardiovascular disease. The current "safe-level" of exposure to radiation that is permissible, is based solely on radiation doses that can cause cancers. However, it is becoming increasingly clear that cancer may not be the only disease that can be induced by radiation. Cardiovascular disease is at the top of the list of potential non-cancer ailments that can be instigated by radiation.

The single cell western blot equipment must meet the following mandatory requirements:

- The system must be capable of microscale separations, quantitation, and immunodetection of proteins in single-cells and must be able to analyse around 1000 single cells per run.
- The system must be compatible with conventional western primary antibodies and standard fluorescently tagged secondary antibodies for immune-probing.
- The instrument must have capability of multiplexing at least 2 target proteins per run and must offer possibility of stripping and reprobing with different set of antibodies.
- The analysis software must perform automatic detection of lanes and protein peaks and offer automatic peak quantification.

Desirable requirement:

- Bench-top footprint would be advantage

The contract can be extended twice by 12 months each.

Read full description

## CPV classification

Immunoassay analysers

Source

Contracts Finder (UK)

[View official notice](https://www.contractsfinder.service.gov.uk/notice/e7c1b7cf-4a7b-48d5-a241-05b493202ee8)

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