Source: https://tendernation.io/opportunities/01a13c35-6b2d-7311-a781-b024e928ff9b/2306jn-chromatography-system-for-protein-and-nucleic-acid-purification awarded GB open procedure Suitable for SMEs # 2306/JN - Chromatography System for Protein and Nucleic Acid Purification [University of Sheffield](https://tendernation.io/buyers/university-of-sheffield) Estimated value £65,000 Published 1 Aug 2019 Deadline Closed 2 Sept 2019 ## Who can bid - Buyer says suitable for SMEs The buyer ticked “suitable for SMEs” on the notice. Anyone can still bid. - Small contract value Valued under £135,018 including VAT, the lowest Procurement Act threshold for goods and services. - Open procedure Anyone can submit a full tender; there is no shortlisting stage. [More contracts for small businesses and VCSEs](https://tendernation.io/opportunities/for-small-businesses) ## About this tender The Bose Lab works on understanding the function of non-coding RNA: protein complexes, and how they regulate gene expression. The group uses a combination of functional genomics, biochemistry and structural biology to probe the mechanism of protein:RNA complexes. The lab is generously funded by a Sir Henry Dale Fellowship from the Wellcome Trust and the Royal Society. The group is also associated with a wider cross-faculty and departmental grouping of labs focused on nucleic acids research within the University of Sheffield as part of the Sheffield Institute for Nucleic Acids; the equipment will therefore be of wider interest to a number of groups within the University. Our work studies how non-protein coding RNAs (ncRNAs) interact with and regulate distinct activities in regulatory enzyme complexes that control chromatin accessibility and therefore transcription in mammalian systems. We have previously shown that direct interactions with ncRNAs are a key regulatory factor for these enzymes. The biochemical and structural biology experiments carried out in the group require the formation of diverse protein:nucleic acid complexes, and their subsequent purification to high levels of purity. We also incorporate a variety of fluorophores into our purified samples. As many of these complexes are only weakly associated or transient, accurate sample preparation requires the ability to operate a number of preparation protocols quickly and reproducibly. Importantly, the ability to simultaneously detect the presence of both protein and RNA components in the sample formation reaction would be beneficial to ensure complexes are formed prior to their use in downstream experiments. The University now requires a chromatography system to support the research into this area. In order to purify proteins, RNA and protein:RNA complexes, the system must be capable of a number of distinct protein purification applications, including but not limited to: Affinity chromatography (e.g Ni/Co affinity, GST, Strep-tag), Anion and cation exchange, and size exclusion chromatography. To preserve the activity and integrity of the purified samples, the system will be housed in a climate controlled cold cabinet operated at 4C. The ability to control and monitor the system remotely would be advantageous. This is an Non-EU open exercise. The ITT can be downloaded by registering and expressing your interest on the University's e-tendering system [in-tendhost.co.uk/sheffield](https://in-tendhost.co.uk/sheffield) If you have any questions or comments in relation to this tender they must be submitted via the In-tend system, this can be accessed at [in-tendhost.co.uk/sheffield](https://in-tendhost.co.uk/sheffield) Completed tenders must be returned through the same e-tendering system. Closing date for receipt of tenders: 2nd September 2019 at 12 noon (UK time). Read full description ## CPV classification Chromatographs Source Contracts Finder (UK) [View official notice](https://www.contractsfinder.service.gov.uk/notice/19e536db-f5a4-426b-b2c7-54a1c5721a7a) Never miss a tender like this again Get AI summaries, deadline alerts and a bid pipeline for tenders matched to your business. Free to start. 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