Source: https://tendernation.io/opportunities/01a13c20-c9b4-7241-ac3a-015b522eb0b1/high-pressure-freezer-lot1-cryo-handling-system-lot2-freeze-substitution-u

closed GB open procedure

# High Pressure Freezer (lot#1) Cryo Handling System (lot#2) Freeze Substitution Unit lot(#3)

[The University of Nottingham](https://tendernation.io/buyers/the-university-of-nottingham)

Estimated value

Not stated

Published

19 Apr 2018

Deadline

Closed

21 May 2018

## Who can bid

- Open procedure

    Anyone can submit a full tender; there is no shortlisting stage.

## About this tender

LOT no. 1:

Essential Features:

### Instrumentation - HIGH PRESSURE FREEZER

The requirement is for a high pressure freezer capable of the rapid freezing of biological samples. This should allow the near instantaneous immobilisation of all molecular components in vitrified ice to avoid the introduction of structural artefacts in samples up to 200 Âµm thick. The samples prepared using this method should then be amenable to further processing or introduction into analytical techniques operating in in high or ultra-high vacuum, including an IONTOF time of flight and Orbitrap secondary ion mass spectrometry, FEI scanning electron microscopy, FIB, TEM, etc.

The instrument should be capable of high pressure freezing of biological samples including but not limited to mammalian and microbial cells (e.g. macrophages and stem cells, bacteria and biofilms) and tissue biopsy samples and sections.

LOT no. 2:

Essential Features:

Instrumentation - CRYO-SAMPLE HANDLING / TRANSFER SYSTEM.

The requirement is for sample handling capabilities under cryo-conditions capable of sample manipulation under cryo-conditions following high pressure freezing.

This system will then enable the transfer of samples into high and ultra-high vacuum ultra-high vacuum, including an IONTOF time of flight and Orbitrap secondary ion mass spectrometry, FEI scanning electron microscopy, FIB, TEM, etc., whilst maintaining the samples in the frozen hydrated state.

Cryo/vacuum-sample transfer chamber should be available under appropriate vacuum and temperature conditions, and within a timely manner, to avoid sublimation and devitrification.

LOT no. 3:

Essential Features:

### Instrumentation - FREEZE SUBSTITUTION SYSTEM

An automatic reagent handling system delivering freeze substitution and low temperature embedding capabilities to samples which have undergone high pressure freezing.

Additional information:

TENDERERS MUST EXPRESS THEIR INTEREST THROUGH FIRST REGISTERING ON THE UNIVERSITY`S E-TENDERING SYSTEM [in-tendhost.co.uk/universityofnottingham](https://in-tendhost.co.uk/universityofnottingham/)

Non-compliant expressions of interest (EOI) will not be considered or acknowledged. The university reserves the right to cancel the tender process at any stage. The University does not accept any liability for any costs incurred in tendering.

To EXPRESS INTEREST:

Please first register on the eTendering system then Login and go to Tenders.

Find the project scroll down to View Details and then scroll down to the Express Interest` button at the foot on the right hand side.

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Once you have registered, log-in and go to Current Tenders/view printable details, then expressed interest in the project.

Once you have registered, expressed interest in the project AND your EOI will be accepted and an email will automatically be sent notifying you (to the inserted publish email address at point of registration) that it has been accepted and a second email notifying you that the documents have been published to your site under `My Tenders`.

Internet Explorer (not Mozilla Firefox) should be used and pop ups must be allowed.

All correspondence relating to this project should only be made through the e-Tendering web site. Click on CREATE NEW CORRESPONDENCE once you have logged on to the Web site and follow the instructions. Select send message via web option rather than `by email` option as this is received by the host company not directly to UoN.

HELP?: Should you encounter any problems with using the Web site (including if you get locked out), contact the In-Tend Help Desk on 0844 272 8810 

Funding Description

### EPSRC

Is a Recurrent Procurement Type? : No

Read full description

## CPV classification

Freezers

Source

Contracts Finder (UK)

[View official notice](https://www.contractsfinder.service.gov.uk/notice/3d72f448-22ce-4f41-84e2-b389faf9e06f)

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