Source: https://tendernation.io/opportunities/01a13bff-6132-7296-93a8-e24e51f31491/sequencing-of-insect-transcriptomes-and-genomes

closed GB open procedure Suitable for SMEs

# Sequencing of Insect Transcriptomes and Genomes

[University of Exeter](https://tendernation.io/buyers/university-of-exeter)

Estimated value

£50,000

Published

12 May 2016

Deadline

Closed

26 May 2016

## Who can bid

- Buyer says suitable for SMEs

    The buyer ticked “suitable for SMEs” on the notice. Anyone can still bid.
- Small contract value

    Valued under £135,018 including VAT, the lowest Procurement Act threshold for goods and services.
- Open procedure

    Anyone can submit a full tender; there is no shortlisting stage.

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## About this tender

The University of Exeter College of Life and Environmental Sciences wish to procure library preparation and next generation sequencing (DNAseq, RNAseq) of insect genomes and transcriptomes for a project looking at the molecular basis of insect resistance to natural and synthetic xenobiotics over the next five years. For this project we will be sequencing the transcriptomes and sequencing/resequencing the genomes of a significant number of insects (genome sizes of 500MB to 1.2TB). We will use this data to estimate gene expression, call SNPs and CNV. Finally we will bisulphite treat DNA samples and wish to have these sequenced in order to estimate the level of methylation in insect genomes of interest. We wish to identify a preferred supplier for this service to establish a framework agreement for sequencing on demand over the next five years up to the value of Â£86,986.

For DNAseq we would require, PCR-free libraries and sequencing of genomic DNA on an Illumina HiSeq2500 using a 100bp or 250bp paired-end metric. For RNAseq we would require TruSeq RNA library prep and sequencing of RNA on an Illumina HiSeq2500 using a minimum of a 100bp paired-end metric. It would also be useful to have the option to carry out PacBio sequencing of genomic DNA.

### Additional information: How to apply

Applicants will be required to register their interest for the relevant tender documents on the University of Exeter's electronic tendering 'In-Tend' System ([in-tendhost.co.uk](http://in-tendhost.co.uk/universityofexeter/asp/home.asp)) The procurement exercise is completely managed via this electronic system and only registering on [in-tendhost.co.uk](http://in-tendhost.co.uk/universityofexeter/asp/home.asp) will ensure that you receive all the relevant information and follow-up document. Registration is free of charge to potential suppliers.

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## CPV classification

Laboratory, optical and precision equipments (excl. glasses)

Source

Contracts Finder (UK)

[View official notice](https://www.contractsfinder.service.gov.uk/notice/a91c2274-adc9-11e6-9901-0019b9f3037b)

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