Source: https://tendernation.io/opportunities/01a13baa-cfe4-7242-a469-b87c2128e4df/rbgkew1309-long-read-ash-genome-sequencing-2024

awarded GB open procedure Suitable for SMEs

# RBGKEW1309 Long-Read Ash Genome Sequencing 2024

[Royal Botanic Gardens, Kew](https://tendernation.io/buyers/royal-botanic-gardens-kew)

Estimated value

£150,000

Published

22 Aug 2024

Deadline

Closed

13 Jun 2024

## Who can bid

- Buyer says suitable for SMEs

    The buyer ticked “suitable for SMEs” on the notice. Anyone can still bid.
- Open procedure

    Anyone can submit a full tender; there is no shortlisting stage.

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## About this tender

Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp.

In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306.

RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.

## CPV classification

Research services

Source

Contracts Finder (UK)

[View official notice](https://www.contractsfinder.service.gov.uk/notice/f0a5cd0e-71a2-47f9-a409-e20abaae72f9)

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